LOCUS BG073401 722 bp mRNA linear EST 12-MAY-2010 DEFINITION H3122B10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3122B10 3', mRNA sequence. ACCESSION BG073401 VERSION BG073401.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 722) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG073401.1. Other_ESTs: H3122B10-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3122 row: B column: 10 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..722 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3122B10-3" /db_xref="taxon:10090" /clone="H3122B10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 172 a 205 c 206 g 139 t ORIGIN 1 gccgaaagca gaatctcttt attatcaaac agcagtgaca ggatagctgt cacacacaca 61 gactgggagc caggggagcc agcggctgct ggagaaccca caggacctca caccagaagc 121 atgtccactg ccccttgctc cacctcttac cctatttctg ttctaagagc cctggactca 181 cagaggggac accaggaatg ttttgtaggc agtgaccatc ttccggggca cagagaacat 241 aatcccagct gggctctggg acctgcaggt ggccacagag caccgactgg ggttacatgg 301 ggcagatggt gctggcccag gctgctcctc tgggctcaca tctgcccatc accacttcca 361 gcccttggag tcccaagcag taatattggc acccagagga ctccacccag gacaggctca 421 ctgaggagac tgggcaggga ttatgggcta ggaccctggg ggtgggggtg gggtccatgt 481 tcacacccag gagaagagaa ggaagagcac cctgtggccc tctgtccagc ttctgatgct 541 gtgctggctc catttccttt gatcccacat caacagcaat agaaaaaagc agccactgga 601 gatgcactga gatttcactc tgggaggaga catggccctg ctccagaccc tcagttagga 661 tcaggggagc ctcctgggtg gttcaggagc cacctggcct ttcctcaaag aaaggaggag 721 gt //