LOCUS BG073173 609 bp mRNA linear EST 12-MAY-2010 DEFINITION H3119E12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3119E12 3', mRNA sequence. ACCESSION BG073173 VERSION BG073173.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 609) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG073173.1. Other_ESTs: H3119E12-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3119 row: E column: 12 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..609 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3119E12-3" /db_xref="taxon:10090" /clone="H3119E12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 242 a 99 c 100 g 168 t ORIGIN 1 aagttttttt ttttttattg gtattcactt cagtaacttg aatccacaga tataagcagt 61 atataaccag agagttacaa gtaaacacag attatacatg caaatttctg ctcacaaagg 121 tcacatgtgc aggtacatga attagaagcg tgcgtcaaga attatggcca aactggtttt 181 gaaatgcaga aatgtaaaat tacatcttga aaatatagat ggtttacaca cttcaaaaat 241 caaatgttgc ttataccaga gatgtatgac agctacagga ttcaagtgac aggcaatagg 301 ttctcaagaa attaatactg gtcaaagaga acgggaatat ttttacattt cactgaaata 361 acattgacta ctagaatata aaacctagca ggaactcaag agaaaaaaat tacaaatcta 421 aagccaattc ttaatatttc ttatcatcta aacagcatga cattaaaaga aaactgcacc 481 tgcaattgaa tggccaatct cacatgaatg aagttctcta aaaatgcaat gcaaccaaaa 541 cagcttccaa agtcaaatga aaagtctgca aggctcagat taaaatgtgg aatgtttcag 601 atgaaaagt //