LOCUS BG073169 718 bp mRNA linear EST 26-JAN-2001
DEFINITION H3119E07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3119E07 3', mRNA sequence.
ACCESSION BG073169
VERSION BG073169.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 718)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Other_ESTs: H3119E07-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3119 row: E column: 07
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..718
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3119E07-3"
/db_xref="taxon:10090"
/clone="H3119E07"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 228 a 140 c 135 g 215 t
ORIGIN
1 gtgaagaata tttttatttt gtttttatta aataccatgt catctttttg tcccattaca
61 aatctgtaga cactacatga atgatacata atttatattc tgtggtttgt caggatgata
121 tatttacaca tgcagttggt atcaatcatg ctacatttcc ttaggagatg cccatgttaa
181 acaaaaggag actgcttatt tactacagga aatacttctg gaggtggggt gcataccaac
241 ctgcacctgc agccaagttt agtactaaaa tattatattt aacttagact tgtgggtcat
301 gttccattgc tttttaacaa tacacataat ttgagcaaaa acaagctttt cccccttttc
361 tccatcacct gttttggtac ttaagaaagt acacagacac accgggataa actggtcaat
421 aataaaagac cacagcctca aaacagaaat agcatttagc agtgatgcca cctaaaaagg
481 cagaacaaag ttgtctggaa gctaaacgga aatcaaaggt cagtggtggg cttcctgggt
541 actgagtatg tgccgctatc acctgcactg cccagaaatg agataccaac ttgggaagaa
601 aatgttacca catgtaagta atttcagttg gaatctgagc tgctgcactg ccattaggag
661 tgtttctctc tgtaacacaa atactctgtg tacagcctat gtgacaaaca gatgtgca
//