LOCUS BG073163 762 bp mRNA linear EST 12-MAY-2010
DEFINITION H3119E01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3119E01 3', mRNA sequence.
ACCESSION BG073163
VERSION BG073163.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 762)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG073163.1.
Other_ESTs: H3119E01-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3119 row: E column: 01
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..762
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3119E01-3"
/db_xref="taxon:10090"
/clone="H3119E01"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 211 a 173 c 235 g 143 t
ORIGIN
1 agtggtagat cacaccttta atcccagcac ttgggaggca gaggcaggag gatctgagtt
61 ggagaccagc ctggtccaca gagtgagttc caggccagcc agggctacac agagaatccc
121 tgtctcaaaa aaacaaaaca aaacaaagca aaacaaaaaa acagatcaaa gcccaaccaa
181 acaaaacaag taagggtcct acagtccctg gtgaaggaca ggcatgaaca cttggacctg
241 ggttggtgac actcctacag gacattctga aggctgcaga tgtgaactgt ccctgggtgg
301 cctgaggaag agaggactgg atgagacatc atggtccctg agatgggggc aggcgcagag
361 aaaggaatga aagcaaaggt ggtttgcagg cttggggagg gaggaagggg tgaactccct
421 accatcccgg cccctggaag gcttcatgcc tagaggataa ctccaggaat ggaacttggc
481 tctgagagga aagggacact acccgtgtat ggctgttgtg gggttctctc ttcctctatt
541 cagactggct atagatctgt ggtgagcaga agccctttgc cagggctgga ggggatacac
601 tcgagcctgg ctaaacttcc tgctctgcaa ggtgggggct atctgtggcc tgctcagacc
661 cctgggagtg gtacagctgg tgcgaaggga atgacagtca taactggcaa gaagcagtga
721 gtggtctggg agactccaga caccagcaat ggctggctct ga
//