LOCUS BG073060 559 bp mRNA linear EST 26-JAN-2001
DEFINITION H3118B10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3118B10 3', mRNA sequence.
ACCESSION BG073060
VERSION BG073060.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 559)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3118 row: B column: 10
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..559
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3118B10-3"
/db_xref="taxon:10090"
/clone="H3118B10"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 214 a 117 c 98 g 130 t
ORIGIN
1 aggcagaaaa aaaccctata ttattatcaa taacagtacc acaactgtga ccttcatgat
61 taatattcac tcctaagaat tttcatttgg caccagatgg catgttcgag agaaacaccc
121 tgggatgtct gaaagcagac atgggtttgt tagcagtcac aggagaattt aacgggagct
181 ggggtgtgta aaacaggctg aaaagtctta agtcatcatc taaacaaagc actgacagtg
241 ccaagcatta ggtcagacac taaaatgact gatataggct catgtggttt ataaaaccta
301 taaaaagact acaccaacaa ggtccctgtc accctgtcag agttcagaca ctaaaacagg
361 aagcaacatt ttagtttaaa acctcagaag aatcaagatc acttcacatg agtaaaaata
421 cctgaaagca aacattttta aaagctccaa aacccaaaat ataaagaaaa aaaaaaggat
481 cagaagactt gatcaatcca cggcatcaca aaacggctgg ttaatctcta tctcccctcc
541 acattaagca tctcatgga
//