LOCUS BG072475 681 bp mRNA linear EST 12-MAY-2010 DEFINITION H3110H11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3110H11 3', mRNA sequence. ACCESSION BG072475 VERSION BG072475.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 681) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG072475.1. Other_ESTs: H3110H11-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3110 row: H column: 11 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..681 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3110H11-3" /db_xref="taxon:10090" /clone="H3110H11" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 156 a 174 c 173 g 178 t ORIGIN 1 atttttcatg atagtgtaaa ctgaatccaa ttcctggccc ccagagcaaa caagctacct 61 tcagaggcaa gaacatccaa ctgctgagat gcagcaatcc catcatgccc tgcagctcaa 121 aaaaaaaaaa agtgaacagg aagtggtcac tggatattgc tgctattact gccattgctg 181 tccgtgccgt tagtctctga ggcgagagct gtgttgatgg agttaaggtt ggcgtcagag 241 ggcatggcgt ctctgcgagg tggcatcctc tcgttaattc ggtctaagcc cttggcctcc 301 tcgaagctag tgatcttatg ttgtggtgaa aatcctttga tggcgctttg cagagtctgt 361 ttcccgccag agagcactcc gctggggagc atgccagttg gggtcaggcc cttcagtgtc 421 aggacactct cactctcttc tctgagaact gagaacactc tggccatttt gcctattgct 481 cggatcttgt ttctgatgac ttccttccgg gctgcggccg tggctccgtc aaatccatct 541 tcttctgacc ccagttcatc gtcggagcag atgttgagaa cattgaccag catctcagtc 601 actttctccc caacaaatgg cagcgaccag gtgaaaacat ccatgaaatt tgggagccag 661 tacggatgcg gggagcagtt g //