LOCUS BG072469 616 bp mRNA linear EST 26-JAN-2001
DEFINITION H3110H04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3110H04 3', mRNA sequence.
ACCESSION BG072469
VERSION BG072469.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 616)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3110 row: H column: 04
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..616
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3110H04-3"
/db_xref="taxon:10090"
/clone="H3110H04"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 97 a 60 c 408 g 51 t
ORIGIN
1 gagacagggt ttctctgtat agtcctggct gtcctggaac tcactttgta gaccaggctg
61 gccttgaact cagaaatcca cctgtctctg cctcccaagt gctgggatta aaggtgtatg
121 ctaccaccgc cggatagtcg agagcgggag gcgggcggtg ggaacgagga gggggggggg
181 aacgaggcgc ggatggagga ggtggatggg gtggttgtga gcggagatgg agagggaggg
241 ggcgggcggg ggcgcgaagc ggggggaagg ggagagggga gggagagggt gaggcagggg
301 gggagggggc ggggggcgga gggggaggtg gggggggggg aggaggggga gggggggggg
361 gcagtgggag gaggggggga gggcaggggg ggaggcgggg agggggggag agggggggag
421 gaggggggcg gggaggggag ggggaggagg gggggaggga gggggggggc aggggggggg
481 gggggagggg gggggggggg ggggaggggg ggggggggga ggggggggag ggggaggggg
541 aggggggggg taggggggtg ggaggggggg gggggggggg aggggggggc ggaggagggg
601 agggaggggg gggggg
//