LOCUS       BG072421                 729 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3110C11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3110C11 3', mRNA sequence.
ACCESSION   BG072421
VERSION     BG072421.2
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG072421.1.
            Other_ESTs: H3110C11-5
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3110  row: C  column: 11
            Seq primer: -21M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3110C11-3"
                     /db_xref="taxon:10090"
                     /clone="H3110C11"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          149 a          180 c          210 g          190 t
ORIGIN      
        1 ctgagtgacc ttcagtaggc agagaacatc agttgaggag aagtcacaga gtttgtattt
       61 cccaggtcag aggctggcag gtgcctggtt ggtgacctgg tgacagctgg aggattgaga
      121 ttctgcagtc tcacaggtag agacacacat aaaattcttc ttactcctca ctggaatgat
      181 gactacaagc agctgtgagc atcacaaaga acagagccca tggggaggcg acggtcatgt
      241 ggaggaaact gtggggtgct ttgtggtact gtcctgtgcc atgagcctcc ctttagggct
      301 gctgggccat cacgggtttc gcgaagatgt gtctggctgt ccctctcctc ccgttaggtt
      361 cctttgtgtc agcgagccac ggtacactga tttccgatca aaagaaccac tgtctacgcc
      421 ttggctgctg ggagaatact tcctcctcag gagagagggc acagctgttg ttgccttggg
      481 catcttggct tggtcatcag ggtctgcagg tggagtcttt gggatcctgt caagtttctc
      541 agtaacgcgg ggaagaaaga tcgggatgcc tgactttctc cgaggttttc ccgagggctt
      601 cactgtgagt cctgcggctc tcagagctgc gatcctggac tggatgtctg agatctcact
      661 ctcagcctgc tgaacttcag aggctgtcat ggccactcta tcctccatct cagagagctc
      721 ctcatctgt
//