LOCUS BG072398 834 bp mRNA linear EST 12-MAY-2010
DEFINITION H3110A12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3110A12 3', mRNA sequence.
ACCESSION BG072398
VERSION BG072398.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 834)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG072398.1.
Other_ESTs: H3110A12-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3110 row: A column: 12
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..834
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3110A12-3"
/db_xref="taxon:10090"
/clone="H3110A12"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 294 a 180 c 146 g 214 t
ORIGIN
1 ccgagggaaa ataagttttt aatatgttct ttcctccata gcagcaagct cctaccaagc
61 ttttcttagt gcaaatcctg taggcttgtg tcacatacag tacacagaac aacacatcac
121 accaccacag atgcttccga gcagagatac tcctcaaaaa tttaaaacta tacaaagatt
181 tctgagcata aggtcctgcc tggagagttc aactagagcg accctcctag ggccgtttca
241 ccgttaattt aaaagtaggg gacaaaaggt gcccagaaag gaaattaaat tccccgcgga
301 gcataaaacc ttgtacaacc catttgcctc caggatctaa tagcaaattt cactccacgt
361 cattgacata taccaaatac agatgcatga agcttgggtc ctactctata ccaaaatacg
421 atatatacac ctcccactgc aaaaggaatc tgatacctag tctttataca aagctgaata
481 ttttcttcct caaaatcaag taaccacaaa gtaaaataaa tggaatattt ttttaaaaaa
541 aaaatcatac agagaaagtt aagttttgag agacagggcc agggtctttc attgtcttct
601 cttacaatgt agatttctca gtagccactg tccccacagg aatgacaatt gatctttaaa
661 aacagagcct ttttaataca gtttatacag cacaagtcca caagtcactt gagaaacaaa
721 caaaatagag atcattatcc taagtcagaa caagtggggg agagagagcc agagaaagga
781 gtgggaagga agtactttaa tgctatctgt ttctattcag gcttggaaca acac
//