LOCUS BG072283 618 bp mRNA linear EST 12-MAY-2010 DEFINITION H3108G11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3108G11 3', mRNA sequence. ACCESSION BG072283 VERSION BG072283.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 618) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG072283.1. Other_ESTs: H3108G11-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3108 row: G column: 11 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..618 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3108G11-3" /db_xref="taxon:10090" /clone="H3108G11" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 157 a 143 c 141 g 177 t ORIGIN 1 aggctgtaga gtaaaccatc tttatttctt ttggttaaaa acaatggatt tgaatctggc 61 atttctgcgt acgtctcatt ccatatgtac agtacattga tgatgtacac ctagcagtaa 121 aaacaaatat ctgaaaggaa aacacactga aaaagtggct tcaacaaagg tggctgtgca 181 gggcaggcca tgcaccgagc acaggaggct cagtagaagc agacagtatg cagaaaggcc 241 cggccactct tctcctcgaa ctcctgcagc agctcgtcga tttcattctc catgtcctca 301 gtgtgctgga tacactggca gagctcgcag ataaggaatg ctcccagagt ggcctcttca 361 tggttgtcct ggatgtccac attgaccaca tgcactggct ggcaggtctc ctgttctctg 421 gagttcagat cttctacaac ctggtcatag actctctctt cacaagtgag gatcaagtca 481 aacaggtccg tgcagttctg gaacctttct ggacggggct tgatcctttt atttctgtcc 541 aacatatgta aaattccatt ttgtgtatag agttctttgt ctttcctgag gaggtcattg 601 tacatctggt catatgtg //