LOCUS BG072234 759 bp mRNA linear EST 26-JAN-2001
DEFINITION H3108C05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3108C05 3', mRNA sequence.
ACCESSION BG072234
VERSION BG072234.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 759)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Other_ESTs: H3108C05-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3108 row: C column: 05
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..759
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3108C05-3"
/db_xref="taxon:10090"
/clone="H3108C05"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 203 a 229 c 130 g 197 t
ORIGIN
1 ccttcaagag ggaaaatatc agggcacagt cacaatcaaa agcaaaagtc aatctccaac
61 tgtccaatgt ctgggatcca actcacgatc ttctgggctc ctccaagggc ttgggtcact
121 tctccagcca ggccctttgt agcacacgag tcatcctcta ggctccagat gcctgtactc
181 cactgctgct gctgctcttg gtggtcatct catggtactg gcatctccaa aacactgcat
241 gaccccttca gtcctgggcc atcaattgca actgaggctg cactttcacc aatggccttc
301 catggcctct cacagtgcca agcctcagct gctctgctca actccttcat gccttcaaaa
361 ccagtaccac ctgggtgacc cttacacatt accaagtcca gccacagcac aaggtacaac
421 tttggctata tctggaacac agccactgtg ctttcagaaa atacttccca gaagatgtca
481 cctcaatgat gctggtctct tcttaatcac cgctaatttc ttagctccag ctaaccagca
541 tcaatagtcc cagtaatgca aagtttttgc tttagtagtt ctggtatctt gttaatcaca
601 gctgattctt cagccccagc taaccagaac tacagaatct tcacaatcaa aacagaaatg
661 gccctgaaaa gagtctttaa ttttccctct gaaatttcac acaccagagc tccaacttct
721 gcagtggtct caacattatc tccaaagctc tacacaaca
//