LOCUS BG071998 638 bp mRNA linear EST 12-MAY-2010 DEFINITION H3105E09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3105E09 3', mRNA sequence. ACCESSION BG071998 VERSION BG071998.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 638) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG071998.1. Other_ESTs: H3105E09-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3105 row: E column: 09 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..638 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3105E09-3" /db_xref="taxon:10090" /clone="H3105E09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 152 a 185 c 141 g 160 t ORIGIN 1 aataaaaata catctttaat cataataatt attgacccac atcaggctga atgccggctg 61 agataaggtg gcgtacacac cattctgtag aagctttaca gttaactaca gtcagcgctc 121 tccatccgcc aggccgtggc ttgcaggtgg gaaggcagag gtgcagcatg aagagcacac 181 ggcacttgtg tcacaggtga ggaatgcaag ctgaagagcg gccagtcctg tgtcaacact 241 gtaaaagttg tcccattgca atgacactgc aatcccgagg aacgttctcc cagctggccg 301 agtggtctct ggacagaatc tcacagctgg cgagcaggcg agcagaagtc tttctaaagc 361 attagcggcc tttccgcttc ttcattttgc ccccagccac cttgttcctc acaccaatgg 421 cgccctcctt atcatcatca tctcctgcat cctctcgctt ccgtttcttc ttttctccgt 481 gctcccttaa ctccatccgg gcaaacctct gggcctcatt cacacgttcc gtgagcatca 541 tgacctcctc atcctgcgtc ggaaagacag gcagcttctt tccaatcaag tgctcgatgc 601 gctggaagag ttccacgtca tactgtgtta caaaagta //