LOCUS BG071939 685 bp mRNA linear EST 12-MAY-2010 DEFINITION H3104H07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3104H07 3', mRNA sequence. ACCESSION BG071939 VERSION BG071939.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 685) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG071939.1. Other_ESTs: H3104H07-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3104 row: H column: 07 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..685 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3104H07-3" /db_xref="taxon:10090" /clone="H3104H07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 159 a 182 c 166 g 178 t ORIGIN 1 caattggcat aatagtctgc tcttgccaca tgtaattcag ggaggtttgc agtgttttct 61 tagttgtcag aatactgtta tctccatact ggaatgaggt tctctgaaaa gttgcatccc 121 taatgaatgt actttctcag aacgagagag actctgcctt ggctcgatct tgctgcttcg 181 aggcctagtg gctttgctgt gcactgttgg agctgctggc tgtctgtccc tggccacatg 241 gctgcatgta gcctgactgc cccatgtaca acgctccagc ttcactaact ctcccgcctc 301 gcttggtgta acgcgtctaa cgttgcttct ttcccatttc actcatgtta caggaaccag 361 gaggggttgg gacccatagg tcatgatcga aagtctcaga cattgcctgt ttcccgtaac 421 agaacaggaa tgatgcatgc cagattgcag cagctgggca gcctggataa ctctgtcact 481 tttaaccaca gtaagtccca cgccaaacca tcgtaaccgc caagccatct cagaagggag 541 agcccctgcc acactgtacc gcgtcaccaa cttgattttt cttccagagc caaacaagta 601 ggaagaagag ttccctcctt ggaggagagt cctgtgcagt gggggggtag cagaatgtgg 661 ggccaaatca actacagcac ccgtc //