LOCUS BG071918 750 bp mRNA linear EST 12-MAY-2010
DEFINITION H3104F07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3104F07 3', mRNA sequence.
ACCESSION BG071918
VERSION BG071918.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 750)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG071918.1.
Other_ESTs: H3104F07-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3104 row: F column: 07
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..750
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3104F07-3"
/db_xref="taxon:10090"
/clone="H3104F07"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 242 a 173 c 172 g 163 t
ORIGIN
1 acaaaaatac agatttattg tgtttcaaca ttcttgcaac tgtacagaga ctgagataca
61 cagtcttcat gttaaatata aacaaacacc aagaatatta taaaaacaaa ttgggaaagc
121 tttcaggaaa tctttccaag ctacgtcctt attaaactgc aatggtgaga ggcgatctgt
181 cgtaacaccg tcatagtcta cagtggctgg ttagcgctac ggcagcaaca gagaagaaag
241 caatgtaatg ttctgatggc tcagttcggt aatcccagca cttgggaggc tgaggcagga
301 ggattgctac aagtctggaa gtagcagagg ccacaccatg agttctaaac tgggatagga
361 tacagaataa gaccctgact caaaaaccaa aattaaaaaa aaacaaaagc aaacaaaaca
421 gccatggttt tgatgtatcc acaggccggc aagatgtgca gaggccacgg ctgtgcacgt
481 gacctgagaa gtcaccaacc gaaagacaaa tgatccacga tgcttgctgt cttccatggt
541 gggaaggcgt tgtcctaacc ccatgaggac acgctccaac aacaaaacct cagaggcaga
601 ctgatggact cctctcagtt cccagccttt gtcctcacgg ggcacagaac acatctcgcc
661 aggtcctgac agcgcagttt gatgcaaagg gaccactgtc tctgtgtccc ctacatttca
721 acatgggctg atagagtctt acaaggaggg
//