LOCUS BG071916 775 bp mRNA linear EST 12-MAY-2010 DEFINITION H3104F05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3104F05 3', mRNA sequence. ACCESSION BG071916 VERSION BG071916.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 775) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG071916.1. Other_ESTs: H3104F05-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3104 row: F column: 05 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..775 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3104F05-3" /db_xref="taxon:10090" /clone="H3104F05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 121 a 242 c 188 g 224 t ORIGIN 1 gtttttaaaa aagtttattg gtgatgattc agaaagggtg tggggtggga aggctggagc 61 cggggctacg tgggccgccc ctcatcttcc tgctgcatct gggcaatcag ctgctggcgc 121 tcagctgcct ggcgcatgcg catcatgacg agactctcaa agtccctttc tgacagcact 181 gagccctttg acagtcctgg ggggccgggg tccactgtgc tctggggctg tccctccaac 241 caggaaacct tcagagggtt gtcagccagg cccacttcat ttctgacagc cagctccgca 301 gctctgacag ttgcaaactc cactatggca ttgcctgcct tctttctgga aagtaccagg 361 ttgagaacct ccccatactt ctgtaaaagc ctcaggagga catctctgga gtagccacct 421 tgggactcat cctccttctt gcacttccac tttagcttta gtttgggggt tcctttgcct 481 tcagtatttt ctgttcttcc tctcagcctc tgttctcggt cctggcggat ctgctcctgg 541 atcagcctct gctgctcttc caactgacgg gaaccctctt ctcgaagtcg agcaatctcc 601 tgctctagtg tggtggcgct cctgctctcc tcctcttcct cactcccatg ggcctgggcc 661 tgccgctccc tggcctccag atcaagtttc actttcttcc tgttctcgtc aagtctctgg 721 gtcctctctg ctgcctgctt cttggctttc ctaaccttgt catatgcagc cctgg //