LOCUS BG071862 602 bp mRNA linear EST 12-MAY-2010 DEFINITION H3104A04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3104A04 3', mRNA sequence. ACCESSION BG071862 VERSION BG071862.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 602) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG071862.1. Other_ESTs: H3104A04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3104 row: A column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..602 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3104A04-3" /db_xref="taxon:10090" /clone="H3104A04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 157 a 145 c 141 g 159 t ORIGIN 1 gtcataaaaa aaactttatt tattcagctt ttgcttaatt ttttttttca agacagggtt 61 tctctgtata gccctggctg tcctggaact cactttgtag accaggctgg cctcgaactc 121 agaaatctac ctgcctctgc ctcccgagtg ctgggattaa aggcatgcac caccacaccc 181 agcataattt ttgagattgg atctcactat gtaggctagc ccaggctggc ctggaacttg 241 caatcttcct gcatcttcct cccagatgtt gggatgctag gcatgtacca ccacgtccag 301 ccagaatttc tgaaaatacc agccccaact caaaacctac aatcagttca atggactgaa 361 attatatgaa agacgaatgg tgaccattgg gctgtaagag tatctatctg aaccttagag 421 tgtgagagtg ggtgacctca gggtatagcc tgagaagctc agagtagagg agaggtgcta 481 ggggccagag acagagacac actgggcctt tctgcttctc tcagggtcag tagatttcag 541 cggagggctc ttctcctctg aatagggcag ggtcttcaag atacaaggac ccccaaagtt 601 ct //