LOCUS BG071838 791 bp mRNA linear EST 12-MAY-2010 DEFINITION H3103G04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3103G04 3', mRNA sequence. ACCESSION BG071838 VERSION BG071838.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 791) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG071838.1. Other_ESTs: H3103G04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3103 row: G column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..791 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3103G04-3" /db_xref="taxon:10090" /clone="H3103G04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 192 a 234 c 172 g 193 t ORIGIN 1 aaaattctta catctttatt cagccaattc tctcaggcat caggccctga tgatgactgt 61 cacaccccca tcccttcccc acattcagtt gaggccacaa aacattacat acattcatac 121 acttccatca aaaatatctg agggagactc tggctttggc aaacaagaag agggtctggt 181 ccagcaacct ttcccttaga ggaggcactg actgagccac tgtagttgtt gtactggcca 241 gccctctctg accccagggt gtttgtttgg aagtcagaag gcacggtaag agggaacccc 301 agactctacc ttaattgctt gtattattct gagcagctac ccttggctct gatagggcta 361 tcctgagacc cattcatgac tcaaaacaat ctttctccat aggtaaggtc tcttacccgt 421 gaaaatataa ccttgttttg tctgtagagt ctctaagatt ctcacagctg cgaccctgtg 481 acttgagcag ctacgcagag tatgacccag tacatacggg cagagggatg caaagccccg 541 ccccactata tccacaggta gacggctggc tctttccctg ttctctgggc tcatcccggt 601 cagcagagga ggacctccat gcacacgcac tcctcaggcc ccacagggcc tgtcacactc 661 acacggcacg ctctttgcac tgcctgttct cctcctgtca tccatggaga cccaaggtca 721 ccccaaagga caggcgtgca gagcctcctg gatcttctgg cgacaacgag aatacttctg 781 caggatctgg c //