LOCUS BG071779 869 bp mRNA linear EST 12-MAY-2010 DEFINITION H3103B03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3103B03 3', mRNA sequence. ACCESSION BG071779 VERSION BG071779.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 869) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG071779.1. Other_ESTs: H3103B03-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3103 row: B column: 03 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..869 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3103B03-3" /db_xref="taxon:10090" /clone="H3103B03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 203 a 231 c 163 g 272 t ORIGIN 1 gttgcctaca atctttattg gagttataca aaaagaatcc ccaaaagtga aaaaatacat 61 tatgtacaaa accactttcc ctcaggagga aggttctgct cgcctcttaa catgcagtgc 121 ttttaattgt agctgcagac tccacggtcc cctctgctgt ttggctctca gcctgattct 181 cagcctcggc ctccatgttt tctgaaactt cctttccact ggagacagca tccccactgc 241 ctccattcac ctctggctct tgtttagctt tctttgcatc atccttccgg ggactctctt 301 cctctggttt cctcttcttt ctgacaagat gggaaatatc agtcacacaa tttgatgagg 361 aagcgccatc tgttggtttt ctactggcaa tcatggatac tgaggcacca gctccactag 421 gagtgaaacc ggaagtagag ctctccacca gagtagcttt cagtgccagt tcggctacat 481 tcccactccg ctgagattcc tttgcgtctt ctatcttctc tcgaatttca ggtagcagtt 541 ctttcagctc ctcaatttct ttctcatatt cagtaaacga tccttcggcc tccttcatct 601 gctcatggag tacagccatt ctcttttcaa tgacatcgat agatttgcca aactgtgcta 661 ctgcttcatc atactgagag ttgtaaccat aggccaaacc taattggtag tgagtctctg 721 caaggagacg atcgtgagct tccaaatatt gttcttgcag gcttaggcaa gcctgaaact 781 cctccacagc ttggatataa ttctcagatt caacactaac ttctccaagt ttaagatggg 841 cttgtgcagc ataaagctgg gcttctttt //