LOCUS BG071695 792 bp mRNA linear EST 26-JAN-2001
DEFINITION H3102B05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3102B05 3', mRNA sequence.
ACCESSION BG071695
VERSION BG071695.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 792)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Other_ESTs: H3102B05-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3102 row: B column: 05
Seq primer: -21M13 Forward
POLYA=No.
FEATURES Location/Qualifiers
source 1..792
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3102B05-3"
/db_xref="taxon:10090"
/clone="H3102B05"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 192 a 119 c 141 g 340 t
ORIGIN
1 tttttctttc agtttgttta catggcggat tatgctaata gattttcata tactggacca
61 ctcctgcatc actaggatgt agcctacttg atcagggtga tttttgcctt tgatgtgttc
121 ttggatttgg ggaggatatt ttatccagta tttttgcatc agtgttcata agggaaattg
181 acctaaaatt cttatatttt attgagtgtt tgtgtagttt agatatcagt gtgactatga
241 cctcatagaa tgagtttgac agtactgttg gtgttttgtg gaaaaatttg aggagtattg
301 gcattaactc ttctttgaaa ttcagataga aacctgcact aaaaccatct gacccagagc
361 tttgttggtt gggaaacttt taataagtgc ttctaattcc tttggagttc taggactatt
421 tgcatagttt gcctgatcct gattaagctt tggcaataga tatctgtcta gaaaaatcat
481 ccaattcatt tatattttct aattttcttc ttttaaagta aggcaaatga ttcttttaat
541 ttccttagtg tctgttaatg tctgcctttt catttctgat ttattagtta aggtaccatt
601 taatgaattt aactaagggt ttgtcaactt tgttgatttt cttaaagatc ctgctgttgg
661 tttcattgat tttatttttt taagaattat tccttttgtt tctaattgat tgattccagc
721 ccacagttta tttcctgcct tcaatttctc ctctgtgttt gtgtgtgtgt gttttcctat
781 cacatcatat at
//