LOCUS BG071679 730 bp mRNA linear EST 12-MAY-2010
DEFINITION H3101H11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3101H11 3', mRNA sequence.
ACCESSION BG071679
VERSION BG071679.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 730)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG071679.1.
Other_ESTs: H3101H11-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3101 row: H column: 11
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..730
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3101H11-3"
/db_xref="taxon:10090"
/clone="H3101H11"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 125 a 191 c 194 g 220 t
ORIGIN
1 gttgttttga tttttttttt ttttagatac gacttctctg tgtagccctg gctatcctgt
61 actctataga caagactggc ctcgaactct gagagctgcc tgtctctgtc tcctgagcac
121 ggggataaaa ggtgtgcatt gctgcaacca cccagcaaca gtttatgact ataaggcact
181 ttggaacagg cgggaaagga gatggggaca gttaaatctc tcttgagtcc cagcatagtc
241 gtcagttccc tctgggctgg agtctccgtc ttaatgtgac agcgttggtg tgctggctcc
301 aggagaggtg tcagtcagtg gcctgagaag tgccttgttt gctgaggcct atggactgtc
361 ctgaactcag accttggcta caggcctctg gggtggctgt ttctggagca aggccagggt
421 gtctcgtttt tcaatagaac gcaactgctt cttctttgct ttcttgagct tggcaggatt
481 gcggatcact tggacaatct cagccttgcg ctcattttct agacggcgtc tcaggttttc
541 agcccggcgc tctttcttct cctgccggcg cctctgtttc tcctcctcca ggtgtcgggc
601 gaatccttag ccagctttct ctcctgtcgc tccttcatct tccgctgcca ggaggtacgc
661 aggggcttgt cctgaaccat ctgggagaac ctcttcttgg agcggtcttt cacacacgcc
721 cggatttggg
//