LOCUS BG071616 771 bp mRNA linear EST 12-MAY-2010
DEFINITION H3101B11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3101B11 3', mRNA sequence.
ACCESSION BG071616
VERSION BG071616.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 771)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG071616.1.
Other_ESTs: H3101B11-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3101 row: B column: 11
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..771
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3101B11-3"
/db_xref="taxon:10090"
/clone="H3101B11"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 275 a 145 c 115 g 236 t
ORIGIN
1 ggtgtttaaa ttttttaatg aatctgtttt taaaacctcg tatttgtgaa aaaaaaaata
61 ctgtagactc agtttcactg catttagcag ctgcaatagt agtataattt ttgattaagc
121 agttctaata gaatgcttct agaagatatt tattccacat gataacttcc tgaagaagcc
181 aagcacactt aattttcatt tttaaatgtc tacaaaattt taattctcca tttcacaaat
241 catttttcaa aatctagaca ttattcaaat gtatgccagt tattatacaa ataaaataca
301 taaaaagatc aaaaatatac atattctctc agcaaatttt gttacataaa taagaaaaat
361 atgtacagcc agcttttcaa agcccaatca tcttaacact gcacctgtgt atacagaagg
421 cgctaataaa acagtgagat tcttttcatg acacctacag ctattaaaat gcctaagacc
481 aatctgaggg gaaattaagt tggaagatct taatgttgac ttcattttct ccctttagaa
541 tattctgtag atgaccttca ggcttctact agcaaccccc gagacctgac acaaaagtca
601 agagtgtgtg aagaaataca caaccccgga cctttgtact cagacaagtg caaaggtgct
661 ctcaaagagt cagacttaga cacagctgca tggcaatttt ctggctaaga tgagccactg
721 aaaattcaaa ttatctgagt cagaagtgtt agaagaactc caaaccctct g
//