LOCUS BG071207 752 bp mRNA linear EST 26-JAN-2001
DEFINITION H3095H12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3095H12 3', mRNA sequence.
ACCESSION BG071207
VERSION BG071207.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 752)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3095 row: H column: 12
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..752
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3095H12-3"
/db_xref="taxon:10090"
/clone="H3095H12"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 179 a 170 c 200 g 203 t
ORIGIN
1 ctaaaaacat atccatattt tatttcattt aattgggggg ctggggagga ggtcagagat
61 ccacatgtcc ctgtcaccct ccctccaccc ccaaggctgg gattaaaggt gtgcacacac
121 agccagttcc tcatatttta atagtcccca aagttcaacc tggaaagatt aagaaagata
181 agccatgtcc tctaaaggct gaggtcagag gtcagatatc cagaagtggg ccattactgg
241 aggaagttgg ctaccaggca ggtgagtgtg gggaccaaca gagtgggcag gggcaaagac
301 agcagctgtc ctggggcatg ggacttgaac acctggcgtt ttcccagcgg ctgcaggggc
361 ctcgcattgt ccttcatgtt caacttctga tcttcgtcat agtacagatt ttttgagaat
421 tccaggaact ggtttttgtg gatcttgatg ggttgtttgt acacagtcca gatgacagtc
481 tcatcacagt ttggtgtggt tagtgagcca ttgtaacgga agtaggtata cattttggtg
541 ctcgggggaa gcatgtcctg caaactgctc tccctcactg tagaggttga atggggtttg
601 gagatactgg gcagtgcttc caccaggggc tggaagcttg gttcaccttg gctcctacct
661 caatcataaa tgccagcact gcaaacttgt ccttcgagtc ctcttgctag atgtcaactt
721 cttgtgcacg atgtcatctc catggcaaag tg
//