LOCUS BG071175 609 bp mRNA linear EST 26-JAN-2001
DEFINITION H3095E10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3095E10 3', mRNA sequence.
ACCESSION BG071175
VERSION BG071175.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 609)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Other_ESTs: H3095E10-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3095 row: E column: 10
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..609
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3095E10-3"
/db_xref="taxon:10090"
/clone="H3095E10"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 121 a 166 c 197 g 125 t
ORIGIN
1 gcaaaaaacg aaagactttt attgaaaaat atgaaatggc ccgattcatg gtttgcctgt
61 ctctgcccac tgtgagtttc tgcggggctg gcaagtagct gcagtggcca ccccagccca
121 gttcttggga agggcgaggt ttctttgtcc attcggcttg gacagttcct aggcccggta
181 gtcaggtggc agccggagaa ggggagatgc cagcctgtct gtcctcttcc agcctagcaa
241 gcagactgtg tgtcgtctgt caccatctgg aaagtgcggt ggtctcaaac gcgaggctgc
301 acgctggacg ggtggggttc atcgactgct ggtagtggtg acggtacgga ctgcgtgcac
361 ccggcccctc agacgtatcc tgtgcggtcg taggcagtgc cggtaccggt gccagggccg
421 gtggatcgcg gcgcagaata gaggatcttg gagggtgcat acttgtcgcg cggtgggcag
481 gagcaacaca gcaaggcgcc ccctaggaac tgcagcgcgg cggcagctca acccaacgta
541 caacccagct cccatctcgc gcttctgggc ctcggggacc aacggcttat agaaatcccg
601 gatagtcga
//