LOCUS BG071144 729 bp mRNA linear EST 26-JAN-2001
DEFINITION H3095B09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3095B09 3', mRNA sequence.
ACCESSION BG071144
VERSION BG071144.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 729)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3095 row: B column: 09
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..729
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3095B09-3"
/db_xref="taxon:10090"
/clone="H3095B09"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 227 a 173 c 141 g 188 t
ORIGIN
1 aaagttaatg ggctacttta taataaaata ggcatcatac atcctcataa agttaaaggg
61 ctgctgaggc aagcctaagt cacacatgat tttcaagagc aaccaccatt tctcaggtag
121 ttttgacaaa acaaatcaac aaaaacccaa acaaacaaac aaacaaacaa acaaaaaccc
181 ccttgttctt taactgttct ctgggaaagt ttttgtgtct gccgatagca ttgctagccg
241 gtgtctggag gtctaacctg caaacagcgc ggcgagaaca tgttcccatg gtcatgtcag
301 gcaaggtgct tgctcggggc cctagcgact caggatgagc gacacgagga tgagtacagg
361 accccctcca gacaccttat tctgtcaaat aagaaatata tcactaaaaa ttctcattat
421 tttgtttttc aaaaaggtta ttaggttcct tggggttttt tgttgtttgc ttgtttgttt
481 ttgaagtaca agatttcaca ccaccaccac cactccagga ggggtgggca gactctagta
541 agccaaggag aaccccttac cataactctg tccttctgcc tcttgccact agagggcagt
601 gcactgccac gggcttaaaa ttccccaggc tagtaaataa atatgatcag caaatcacat
661 tttcttaggg tcaatttgtt gcaatgaaaa agaacacgac ttccaaataa ggcacaccca
721 cacccacac
//