LOCUS BG071015 850 bp mRNA linear EST 12-MAY-2010
DEFINITION H3093G07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3093G07 3', mRNA sequence.
ACCESSION BG071015
VERSION BG071015.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 850)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG071015.1.
Other_ESTs: H3093G07-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3093 row: G column: 07
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..850
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3093G07-3"
/db_xref="taxon:10090"
/clone="H3093G07"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 273 a 187 c 158 g 232 t
ORIGIN
1 catagttaca atgtcattta tctcactcct gtggcataca ttgagaatat ttaaaaacac
61 tttgatagtc cattgcggat tatggaatgt tacacatcca tttctataaa ttaaatgttt
121 taggaaaatc cacagtttaa aaaaattata caagaataag ctagtatatc acaatcaaca
181 aagaatgaac ctcaagaatt ccaaataata taatgtcagg aactgcttct cttcacagta
241 aacactcact ttacgcaaat cccatttggc ttcatttaat taaaaagcta tatggatagc
301 catggtactt tgaacgaaat attgaaagga gaaaactgaa gaagaagacc accaagccca
361 atcccacgag gcaaatgatg cgtcttttcc cgacatcccc tttggaagtt gctgcttcat
421 ttagaagaac catccccatg gtgatagcag cattcagtac tagaatgatg tgtgactctg
481 ccacaaactg agcctggctg cttccatgaa tgtagctcac ttgtccattg tgtgggttct
541 tatgagcata tggaggtcca cggatatgat tccacatctg gccagacgtc atagcaaaga
601 ctatacacag agataccatg gcccaaccag tcttgttata gataaactcc aagttgttcc
661 tccttagata aagcaagcca ccaaccagtg acactaacag ggccaaagca atggtgcctg
721 agtagttggg tggccggaaa actcgaatat gaacatccgt cctgtcggca atccattttg
781 ctagctgctc agctgcaaat ccaattcgtt gaaggtcaaa agtatcagct ctcttgggtc
841 tgccttttga
//