LOCUS BG070990 753 bp mRNA linear EST 12-MAY-2010
DEFINITION H3093E03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3093E03 3', mRNA sequence.
ACCESSION BG070990
VERSION BG070990.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 753)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG070990.1.
Other_ESTs: H3093E03-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3093 row: E column: 03
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..753
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3093E03-3"
/db_xref="taxon:10090"
/clone="H3093E03"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 231 a 173 c 163 g 186 t
ORIGIN
1 accaatacaa atttattgga atttggaatg taaacagatg tttcgaacaa acaatctaac
61 cttataaaaa tgtgtggcat tattaaatag tttctgaaga aaccctaagt gtgatgtggt
121 ggatatctca agcatgttag gtccccagtg ctgaggcgtg gctgcagcca aggagagggg
181 actagctggc aaaccatgtc agtgaccaat cccacaaatc aaatcagccc tatttaaaaa
241 aaacaaaaaa ccaaaaacaa aaaacaaaat ttagcttctg ctaacgtgaa ggtaacagtg
301 gatctgtaaa gctttgacac ttgctccagg agccccactt cctcccggaa gccatggctc
361 tccctctcca gccagctgca ggagttccag gacagcctta cccacagcta gcagccgcat
421 gcaagagatg tctttgcgtt cacattcatg tatgacctat cagagctcca tctgcccacc
481 atcctgactg cagccagact tgtttgtcac agggttaggg gagggaagat ggtggtcggc
541 ctttttacac ccgtgaggtc tttcctaaat aaatcctaat agaactgttt tcctccctct
601 ttgacctgcc tgaagaccaa ctttccagct atagctatgg tagtgaacaa aaatagtctt
661 tgttttaaag tggagcattg aagtagcgcc ctcacaaaga tgtgctgaga aactgtgagg
721 ttcttacaag gccaggggga aaaaaagcta aag
//