LOCUS BG070970 885 bp mRNA linear EST 26-JAN-2001
DEFINITION H3093C05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3093C05 3', mRNA sequence.
ACCESSION BG070970
VERSION BG070970.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 885)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Other_ESTs: H3093C05-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3093 row: C column: 05
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..885
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3093C05-3"
/db_xref="taxon:10090"
/clone="H3093C05"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 238 a 242 c 161 g 243 t
ORIGIN
1 cgcttttttt ttggggagga aagggtttat tcggcttaca tttccatact gctgttgatc
61 accaaaggat gcaggactgg aactcaagca ggtcagaaag caggagctga tgcagaggcc
121 atggagggat gttctttact ggcttgctta gcctgctttc ttatagaacc caagacaacc
181 agcccagaga tggtcccacc cacaaggggc cttgcccnct tgatcactaa ttgagaaaat
241 gccttacagt tggatctcat ggaggcattt cctcaactga agctcctttc tctgtgataa
301 ctccagcagt gtcaagttga cacaaaacta gccaatataa ctgacccctt gtcaacttga
361 cacacaaaca catcactagt aagcctcaac ccttacattc ttattcatcc ccaaggtcta
421 aataacttta aatgtcccac agtctttaca tattcttaaa atttcaatct ctttaaaata
481 tctcttttaa aaatccaaag tctttttaaa ttaaaagtct cttaactgtg ggcatcacta
541 aaacagtttc ttccttcaag agggaaaata tcagggcaca gtcacaatca aaagcaaaaa
601 tcaatctcca accgtccaat gtctgggatc caactcacga tcttctgggc tcctccaagg
661 gcttgggtca cttctccagc catgcccttt gtagcacacg cgtcatcctc taggctccag
721 atgcctctac tccactgctg ctgctgctct tggtggtcat ctcatggtac tggcatctcc
781 aaaacactgc atgacccctt cagtcctggg ccatcaattg caactgaggc tgcactttca
841 ccaaatggct tcccatggct ctcacagtgc cagcctcagc tgctc
//