LOCUS BG070967 741 bp mRNA linear EST 26-JAN-2001
DEFINITION H3093C02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3093C02 3', mRNA sequence.
ACCESSION BG070967
VERSION BG070967.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 741)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Other_ESTs: H3093C02-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3093 row: C column: 02
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..741
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3093C02-3"
/db_xref="taxon:10090"
/clone="H3093C02"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 180 a 200 c 132 g 229 t
ORIGIN
1 gttttgtttt gtttttcgag acagggtttc tctgtgtagc cttggctgtc ctggaactca
61 ctttgtagat caggctggcc ttgaattcag aaatccgcct gcctctgcct cccaagtgct
121 gggattaaag gcatgcttca ccatgcccag ctgcaaccct tagtttctta ttcatcgcca
181 acatctaaac aactttaaaa gtcccacaat ctttacatat taaaagttca atccttttaa
241 aatatccaat atcttttaaa atccaaagtc tttttacaat taaaagtttc ttaactgtgg
301 gatccactaa aatattttct tccttcaaga gggaaaaata tcagggcata gtcacaatca
361 aaagcaaaag ttaaactcca actgtccaat gtctgggatc caattcacga tcttctgggc
421 tcctccaagg gcttaggtca cttttacagc cctgtccttt gtagcacaca gcttgtcttc
481 taggctccag ctgcctgtac tccactactg ctgctgttct tggtggtcat ctcatggtac
541 tggcatctcc aaaacgctgc tgtcttccac tataacttgg cttcaacaat agcctctcaa
601 aggctctctt catggtgtca agtctcagct cctttgcatg acctcctcag tcctgggcca
661 tcaattgcaa ctgaggctgc accttcacca atggccttcc atgggctctc acagtgccaa
721 gcctcagctg ctctgggtga c
//