LOCUS BG070926 819 bp mRNA linear EST 12-MAY-2010
DEFINITION H3091G10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3091G10 3', mRNA sequence.
ACCESSION BG070926
VERSION BG070926.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 819)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG070926.1.
Other_ESTs: H3091G10-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3091 row: G column: 10
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..819
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3091G10-3"
/db_xref="taxon:10090"
/clone="H3091G10"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 278 a 167 c 140 g 234 t
ORIGIN
1 gcagactctc gtcttgaaaa acaaaaaacg aactttaaac atagctttat acacctttaa
61 tctcagcagt cagaaagcgg acaggtaaat ctgaggttta gcatggtcta taaagtaagt
121 ctcaagacag ccaagactac atagagagac cctgcctcaa aacaaaacta aaaaagtaaa
181 ctctgtcact gaaaggactg accttacttc tacaagcacc cacaaaaaat cctcaagtag
241 tatcttactt ttatttcttg tttgtgcgac aaaatttcct aacaaagcaa gttataaaag
301 aaagggttta ttttagctta caatttcaga ttatggtcta ccattgcatt caaggtgcca
361 tccagtctgg agtagatacg gatcacatgt gtgcctacat ttgagtgctt ctttaaatgg
421 tccaggagtc aaatactggg aatagtgata tccactttta atctggtaat caatttaatt
481 aacttaaggc aatctcctac agacatgcct ccaggccaaa catagttaga taattaattc
541 tctgtaaaga tttctgagga attctgagag ctgaactcaa gcaatctgct tacctctgtg
601 cccctcaagc actagaatta aatgcccagc acagctctta tccatgtcag agaagtctac
661 attaaaacta tcacagtcac tttttgttct aaacatctga ggcaaataga aaaatctgaa
721 aatggacaca ggagttataa ctcttgggaa cattctgcag tttcttttta agattctgtt
781 ggaagacctc agtaatgact gaaactcaat gagcgcttg
//