LOCUS BG070923 769 bp mRNA linear EST 12-MAY-2010
DEFINITION H3091G07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3091G07 3', mRNA sequence.
ACCESSION BG070923
VERSION BG070923.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 769)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG070923.1.
Other_ESTs: H3091G07-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3091 row: G column: 07
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..769
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3091G07-3"
/db_xref="taxon:10090"
/clone="H3091G07"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 208 a 147 c 174 g 240 t
ORIGIN
1 agtagcatgt taacaaactt tactatattg ataaaaataa tgaaacaaaa ctgtgcttta
61 agagagccag ggccgaggag atgctgtgta agactgagga ccctggttca atctccacta
121 gcctgtaaga tggatatgtt acaggaaaaa caacaagatg tatttaaaac gtcccaacgg
181 tcctcttcag gtgttagtat tctggtgatg taattttctt cacaatttcc tggacattag
241 atcaatttct ttgcaatgat gaaatacttt tacaatcaag acaagaaaag aaagctcttc
301 ccactcaggc agtatttacc ttgatcgttc cgagatccat gggatttttg acaacgtcat
361 agtagttgtg gagtcccaaa gcatcagcat ccacaggatt atagaagggc catgcatacg
421 gcaaatgttt cttggcaagc atttctttaa gaatctcgct gcagtgcttt aattgctccg
481 ttactttgac tgtttttaaa actttgtgtt gttgctgaga gtctggcaaa acgttcttta
541 ctgtattttc ttttactggc atgttcactg gctttgtttc tctaagtgtc ggaggagatt
601 cgctacttgc tttagctatg gaagtggtag gagttgttgt gtctgctcgc ctcttcacac
661 cctttgtaat ttggaccaga gactgcgagt cacagatcgg gggagcttct tgtgccatgt
721 ttaagggcga gagagagatg tcaggaagcc cagaaggaat ctcttgccg
//