LOCUS BG070909 782 bp mRNA linear EST 12-MAY-2010
DEFINITION H3091F05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3091F05 3', mRNA sequence.
ACCESSION BG070909
VERSION BG070909.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 782)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG070909.1.
Other_ESTs: H3091F05-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3091 row: F column: 05
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..782
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3091F05-3"
/db_xref="taxon:10090"
/clone="H3091F05"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 201 a 122 c 202 g 257 t
ORIGIN
1 cctttttttg ggtaggaatt taatgtggtc atatgtttgc aagtaaaaat ctgaataatt
61 tatgaattag aaaaattagg taattgttgc tgtttatctt gtttttgtga ctcaatggtg
121 tgactgttag atctctcatg aatataagaa gtcttgtctg gaacgtataa gaaatgagtg
181 attataaaaa gtctgtgtgt gaactctaat gcctgaaatc ttttttatag tagtatttcg
241 tcttaaaatg ctgctgaagg gagattattt gtaattattc aataaacgtt atttatatga
301 gcagtttgag taatctgaaa ttataagcta ggaattatga acactagagt agaccagagt
361 ggcagagctt cataagtgtg ggtgctcatg ctatttgcta gaagaaagta aagtgattga
421 gtcacgtcat aaaagtgcaa ggtttgcttc ctgtgggttc tctctgttct gtaacaatgg
481 gaactgtagg gtctaactgc acatctcgag actgcagaca gcgccgtggg ctatgtgctg
541 gcttagtgct ttccttctcg ttgtgtgtgc gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
601 gcttgtccag aggtcaacct caagtcttat ttcttagaaa tgccactttg gccgggtggt
661 ggtggcgcac gcctttaatc ccagcactcg ggaggcagag gcaggcggat ttctgagttc
721 gaggccatcc aggtctacag agtgagttcc aggacagcca gagctacgca gagaaatcct
781 gt
//