LOCUS BG070867 803 bp mRNA linear EST 12-MAY-2010
DEFINITION H3093B02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3093B02 3', mRNA sequence.
ACCESSION BG070867
VERSION BG070867.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 803)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG070867.1.
Other_ESTs: H3093B02-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3093 row: B column: 02
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..803
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3093B02-3"
/db_xref="taxon:10090"
/clone="H3093B02"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 182 a 203 c 211 g 207 t
ORIGIN
1 ggtatagata gcccacagtg ggctggaaag gatttatttt aaggcccctt cagaacaggt
61 tctctacagc cctgtggcca ccagaccagg aggggctaat ttatcccaag gactgaggga
121 ggggctgagg tctagcttgt accgtgacaa cttttgacag agatgctatt ctttccgaaa
181 aatcaggcac ttgttgttgg ctggcatgtc cacctggaaa aacgctttgc tggagtgact
241 tggttttttt tttgttgttg ttgtttttgg ttttttcgag acagggtttc tctgtgtagc
301 cttggctgtc ctggaactca ctctgcctcc caagtgctgg gattacaggc gtgcgccacc
361 atgccaggct ggagtgactc ttaatcacag ggaggctccc cacccccaca tcgccctgcc
421 taccatcctc tccaggacca agccactggc ctggcctagt tcctccagaa ggactgtgtc
481 acgaagcccc cactctgggt tcctgcagtt ggggaggaag gtagaaggca tttgcagagt
541 tgaggttatg atcccgccta ccctctctct gagccaagct acctcctagc cagatctgac
601 ctgcatctga gggtcaggtc aaagtccacg ttgctctggg gagagatcct cccattgacg
661 gcatagggct gtgagaacaa gacacaatac ataggcccaa agtcagtcgt tcccacacag
721 ctagtttgca ggataggatg gagttctttc cataggagta tagaaagaat aacttttttc
781 tgcactcaag aaggaaaagg gct
//