LOCUS BG070851 611 bp mRNA linear EST 12-MAY-2010
DEFINITION H3092H06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3092H06 3', mRNA sequence.
ACCESSION BG070851
VERSION BG070851.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 611)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG070851.1.
Other_ESTs: H3092H06-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3092 row: H column: 06
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..611
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3092H06-3"
/db_xref="taxon:10090"
/clone="H3092H06"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 222 a 125 c 111 g 153 t
ORIGIN
1 aaaatgaaaa cctttattga taatttagga ttgcaacaga gagttccaca aaaccaaaca
61 cgtccccaga caatggtgta gaaagctgta gcacatgggt cactgttcat taatattagt
121 gttaacaact taaatactta gtataaaaca ctaaataacc cttcaggcca gataattcct
181 aaagactgaa agtgaataaa aataacgaca attgggtatg gggttcttgc ctgtaatcat
241 gacattcatc aggctatact ggggggatca aattcaaggc ccacctgaca cacaaagtga
301 gatactaaca atcaccaaat tttaaaaaat tgtaacctga aatagaacaa ttttgaggaa
361 agagctctgc ctcagtaaac ttaatataaa caggtcttct tgttaaatga tttaagacat
421 atcactacag agcaggttag cacacctgct gaggcttctc tcccttccca ggcctgaggc
481 aaccagaggt ttaaacttaa acctaaacac ctagcaagta gcagtcccat ggtagagagc
541 aacagaaatt taaattcaag gtctcaaggc cacaagacct gcttagccgg agttaagctt
601 ttggaactat c
//