LOCUS BG070834 764 bp mRNA linear EST 12-MAY-2010 DEFINITION H3092F09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3092F09 3', mRNA sequence. ACCESSION BG070834 VERSION BG070834.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 764) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG070834.1. Other_ESTs: H3092F09-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3092 row: F column: 09 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..764 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3092F09-3" /db_xref="taxon:10090" /clone="H3092F09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 185 a 173 c 180 g 226 t ORIGIN 1 gtgacctggg gttgaggggg tggctgtcct taagcaccct tctaaccctg aggcttttta 61 catttcatgt ctttcggcct gagagagctt tgtcagccag tgtaggttgg gagctgattc 121 ggagtgagct gtgatgtccg tttgtcccta atatgtgtac agggtggttg atgagtcagt 181 ctggtggcca ctgaaagatg aaatggctat tattccttct tgttttagaa agaagtttcc 241 atggtttata gaacaattac tcaacttggt tttcagttct tatttgtacc agtgcaactg 301 aataagtgac agcccccttt ccttctgttt ctgatgtctt tgtctctgtt ctccatagag 361 agcttcactt cccaaattct tcattagagg acatctgaac tccactaact gcgctatcac 421 gcagcccctg acaggagagc tggtggtgga acactcggat gctgctatcc ggagcataga 481 gcttcagttg gtccgggtgg agacctgtgg taagacttct agagtatcta ggtatctaca 541 cttgtactgt cagccgctaa aacacccctt cctgtactct taacatggca gggcacatca 601 catcataaca gagctattct gatggcccct gtggcctact caccccaaaa ttcccacttg 661 cagataatca ctgaaatact aattaggtcc ccagcacctt aaatcttgga agcatggtaa 721 aaccagtgtg gtgatgtttg agaacatgca aagtagaaga gaag //