LOCUS BG070826 808 bp mRNA linear EST 12-MAY-2010
DEFINITION H3092E10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3092E10 3', mRNA sequence.
ACCESSION BG070826
VERSION BG070826.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 808)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG070826.1.
Other_ESTs: H3092E10-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3092 row: E column: 10
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..808
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3092E10-3"
/db_xref="taxon:10090"
/clone="H3092E10"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 237 a 148 c 177 g 246 t
ORIGIN
1 aaattttaaa aaaatttatc tttgaggaag ggagaacttc ctgccactct cactgatctg
61 atgactccac ttcctgttat ccaggctcct agttctttaa ctgcaaatct gttctacatt
121 ttcttgtatt cttcaatacc tggctcaaag gtctttatga aggtgtctct gaaacctgca
181 ggcataggca gagtgagttg gtggtttggt tttgtgtttg gttggttgtt ttttgagata
241 gggtctagtc caggttggcc ttgcggtcac tatgtagttg aggataggag ttcacacttt
301 ccaatggata tttagtgagt accttgattt gtgttggtaa ccctgtcgtt tactggttcc
361 ttaaatgttg gattaaaaca aaaacaaaac aaaaggatat gaggacattt cttccatgtg
421 acaggttact ttctgtctgt atttcgtact aatagatatt cattcaaatc gcctggtgca
481 gtagaaggct aagcttttca gccagatgaa cctgagatca gatcctggcc cgccccttac
541 tgactgaccc tgaacaagtt tctgtgtctg caacatgaag atagcaatga tctctgcagt
601 gacctctgta agactgaata gatgcattgt aggcacactg cgaatttgtg ggaaaatgct
661 ttctaatcta taaagaacta caagaactca agagataatg atgctgtgag ccggtggaac
721 aaaaaagata gaaataagac aagcaaactt gcaaacaagt aacaattgtt taaacataag
781 gcaagagcag caggcaaaca aggtgact
//