LOCUS BG070820 751 bp mRNA linear EST 12-MAY-2010
DEFINITION H3092E04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3092E04 3', mRNA sequence.
ACCESSION BG070820
VERSION BG070820.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 751)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG070820.1.
Other_ESTs: H3092E04-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3092 row: E column: 04
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..751
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3092E04-3"
/db_xref="taxon:10090"
/clone="H3092E04"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 187 a 171 c 136 g 257 t
ORIGIN
1 gcagaaaggc ttgctgggga agcagcaagt actgctgttg aacccttcac ctctgggctg
61 gcctctgatg acaaggacgt ttcatttact gcaggttctc ttttcttgct cgcttgcttt
121 ctgtgagcat caccatttct atcagaattg ggactaagtc tctgggtctt ttcactactt
181 aagtctatgt cctttctttg ttttttctga gaagagtgac taacaccagg actgtttttc
241 ctcttcttgt gaccattacc cagagtttca ggcaactcta aacaatccaa ctttgatgga
301 ggctccgact ttaaattatg tctcttgatt tcacaggagc caggagactc tgaggtcaaa
361 tcaatacaag tttcttcaac aatactttct aaaggactgt ttgcccttcc catctttcct
421 tctttgttgg tatcctcatc tatacatatt acctggcatc ccataccttc aacagctaac
481 acaggttcac taccttcatt tttactctca cttgaagcat catgtgtcaa atcaataaaa
541 gcatctatag tatcaggctg taaagaacta catgttatct ctgcatcttg atctaagttg
601 gtttgagaac attccaagtg atcaacattt aaaactgtta cttccattag ctcccccaag
661 cttttggtct cagtggctga atctttcgtt agatctatgt gtgtatcagg aagatgatta
721 tcaagagagt gtgggatttt ctccattgaa g
//