LOCUS BG070801 783 bp mRNA linear EST 12-MAY-2010
DEFINITION H3091C10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3091C10 3', mRNA sequence.
ACCESSION BG070801
VERSION BG070801.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 783)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG070801.1.
Other_ESTs: H3091C10-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3091 row: C column: 10
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..783
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3091C10-3"
/db_xref="taxon:10090"
/clone="H3091C10"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 255 a 181 c 121 g 226 t
ORIGIN
1 atagattcac taaatttatt ttaaaaatca taaaacgttt cttacaaaag agcattacat
61 tctgcacact gctctgaaca aatgccaggg acatgtggac tattactttc ctccctgtcc
121 caccccccaa tgttacagtg accacaaagt aaggtgttca caataattac atggggggaa
181 ttttttttaa aaccaccaac aatgaacaaa aaataaaagt cactcactct gctgctgttt
241 caaaatttca atgttagttt ttgcatgccc tccccccaac cctgtttgta aggaactaaa
301 acattacatc tggtgaacag caaagatttc actacacctc aaatgcagaa cacctatgaa
361 gcagaggaat gttggctttt taaacagaag cagataaaaa aaaaagatgc aggactcctt
421 cagttcttca ctagtcttag aaaaactttc cagaatactg cttcacacta taaaaaagaa
481 aaaatatctt gcattagaat ccttcaacat ctgcatactg cttcacactg ttctgcagca
541 aatactgtgc gttctgtatc tggtcctgtg ttcctgtaat ggtaatgatc cgatcttcag
601 atccttctaa aggttcatca attttgattg atgctccaga ttcatgacga atttgtttaa
661 tccgctgacc acctttgcca ataatagatc cagccaaatc tttgggaata gttacttgtg
721 tagtgataat aggtccgcca agatcaccat atgagccacg gcccccatat ccagaaccac
781 cct
//