LOCUS BG070588 554 bp mRNA linear EST 12-MAY-2010 DEFINITION H3089A01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3089A01 3', mRNA sequence. ACCESSION BG070588 VERSION BG070588.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 554) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG070588.1. Other_ESTs: H3089A01-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3089 row: A column: 01 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..554 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3089A01-3" /db_xref="taxon:10090" /clone="H3089A01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 110 a 180 c 123 g 141 t ORIGIN 1 agtttttcaa gacagggttt ctctgtgtag ccctggctgt cctggaactc actctgtaga 61 ccaggctggc ctcgaactca gagatctgcc tgcctctgcc tcccaagtgc gtacgccacc 121 actgcctggc ccctgataac ttctatcatc gctttttggg ataggctgtc cggtaccata 181 gcttagcctg gaactcaggt agctaaggat gaccttgaac cccatgctgt gcccgaggtc 241 tttggccagt cttctcttca ttttcaacac tgtagcaagc tttctgcact gcagggtgca 301 agcagctggc caagccactg gggatggact tggcctgcac ctttctctac tctaaagcac 361 tggctgagcc ctctgcccca tgtctccgtc acaccacaga tgggatgggg ccatctatac 421 acagggctcc ctgccccttc ccagaggaac cctccattgg acacctgcct accacctcag 481 ggtttagtca ctgcactgat ttcattctaa caaacagcca cttacacacc tggcacacta 541 tgcttccggc cttc //