LOCUS BG070521 751 bp mRNA linear EST 12-MAY-2010
DEFINITION H3088C04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3088C04 3', mRNA sequence.
ACCESSION BG070521
VERSION BG070521.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 751)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG070521.1.
Other_ESTs: H3088C04-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3088 row: C column: 04
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..751
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3088C04-3"
/db_xref="taxon:10090"
/clone="H3088C04"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 193 a 176 c 160 g 222 t
ORIGIN
1 ataacgagcc attaaccagc cactttgaaa acacaatgca gaccttcttc tctgctgcag
61 tgcccttcca ccacataaaa acctaaggcc aggccttcag cagcggggca tttacagcca
121 gccccagaca gtttaaggag ggcaatgaac agggtggggc tggaggaaat gaggaccaac
181 aaaaggcagg atgctgattt tctttctgcc tctttttttc ccccttttct tttttccttt
241 tcaaagaatc tcctgctact gtggggtttg aagtcctggc cccactgtct tctgggggaa
301 gtgggggagg gcaagccacc atatgatgag attttatttc tctccctcag gatttctatc
361 caagaaaaca ttctggtcag ccaaatccat gggaagatgt gacttaagtc ttcttcttat
421 actgtaattg gagagggtag cattcgtacc agatgtaatg tgtccacctt caagaaagat
481 gacggagctt tgaacatgac ctctacatta tgatgaaatc tgtcagattt cgaggacatg
541 ttctcctcca cgtctgagtc attgggatcc acgatgtcat caaacggtgg caacattgac
601 ttcttcttct ctgaagtctc actctcaatt ttgacctttc ccatcttgac atgagattta
661 tcttttatct gttttttgtc agcagaacaa gtgagtatca gtgggggcgc actagaaaaa
721 cttttaaata aagcctccga gctactcttt t
//