LOCUS BG070488 758 bp mRNA linear EST 12-MAY-2010
DEFINITION H3087H06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3087H06 3', mRNA sequence.
ACCESSION BG070488
VERSION BG070488.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 758)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG070488.1.
Other_ESTs: H3087H06-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3087 row: H column: 06
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..758
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3087H06-3"
/db_xref="taxon:10090"
/clone="H3087H06"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 145 a 245 c 162 g 206 t
ORIGIN
1 ctttttcacc tctttatttt tttttaactc cagacgcatg gttttcagtg agactatgac
61 cacctaaaag actccaagtt atttgaccct gagcagctcc cgtaggtgtc atgtcagtcc
121 tcacacacct ccctcttcct ggagttgcac agctataacc tacaacctgc tccctctgca
181 ttggtggctt tcacaccact ccatcactga ccgaccgact ctaatgttgc tttctgcaac
241 ctgtaccacc ttccttcaca tctatgtgtc tgcaaccctg acatccccac tgctcacgta
301 cttcagggca gtggtccttt gtttgggggg gggcacttga acacaatgag gggaagagat
361 tctgagtcat ttgggcacac cctgctctaa cccaggactg gcctaaccac acaatcatcc
421 tcttacagct cagctgcttc cttcccaggt cagcagcccc tacccatcac actgcccaac
481 ctggctctcc atccccatcc ccaaagagcc acagtggtct tggggctctt cactctcctg
541 ctgctgaggg aggatgggtt gtctggtctt tctactacct gtcacttggg ttttctcttc
601 ccagctggga gccaggaaac cacccagaga cctggcctgg ttcctttcag ttcatgcctc
661 tagaccctgc cgcagcctac ggtccagggc taggagctgc ctcaagaagc cccggttggg
721 ggtgatgcct cggtggtcct tgactttctt gatggcct
//