LOCUS BG070473 733 bp mRNA linear EST 12-MAY-2010
DEFINITION H3087G02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3087G02 3', mRNA sequence.
ACCESSION BG070473
VERSION BG070473.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 733)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG070473.1.
Other_ESTs: H3087G02-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3087 row: G column: 02
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..733
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3087G02-3"
/db_xref="taxon:10090"
/clone="H3087G02"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 192 a 161 c 178 g 202 t
ORIGIN
1 cattttgtaa agattttatt ttattttatg tgtatgggag ttgtgccggc atttctatct
61 gtgcaccatg tgtcaattcc cttggaactg taggtacaga caatcccatg tgggtgatgg
121 gaattgaacc agctcctctg agagagttgt cagcactctt caccactgag ccatctctct
181 agccccaaat aatggaagtg tgtggatagt ggggtgcctg aactctcctg agtaacttca
241 ggcaacttga gcacacatta ccgtgaaata tcttcatcag cagtgagctc ctgctccatc
301 agtaagaaca cctgcacaag ttctgtgatc atggtaggcc agagtgaggt gaggtgttgt
361 ggagacattc ttaaaagtaa cactctgaaa aacaggaaca cttgagagtg aagggtcggc
421 acctggggca aacggagact ctcaaccaat ctctcttgta tgtctggaag gtacttttgg
481 tactggtcac tttcactgct aagaacggcg aaggccagtc ttttgagaag catggctcgc
541 tgttctagct ccacatcccg gtttgcaaaa agattgagag aactgctctg ggccacggct
601 actcgagtca tcaaatctct gaaggttgtc ttatcatgtg tcatcaggtt gtccatgatt
661 gctctccagt gactaacaca ggaagcatcc atctgaaaga agctgggatc cataaaaagg
721 tcaaaggctt ctt
//