LOCUS BG070408 738 bp mRNA linear EST 26-JAN-2001
DEFINITION H3087A07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3087A07 3', mRNA sequence.
ACCESSION BG070408
VERSION BG070408.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 738)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Other_ESTs: H3087A07-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3087 row: A column: 07
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..738
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3087A07-3"
/db_xref="taxon:10090"
/clone="H3087A07"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 231 a 125 c 193 g 188 t
ORIGIN
1 ggaagaatgt agacttaggg actttggatt tggaaaggag tggaatgctt taaatggggc
61 ttactgggcc atcctaatag gaatatggaa gattttgttg ctgagagtaa tttgtgctgg
121 gctgaccttg cccaagaggt tttaaaaaag tagaatttca gtatgtggaa taaagattgt
181 tttgtggtat tttggtggtg aatgtggcta ctttttcctc taatagaagt aatctgcctg
241 aggctaaggt aaagatacca agattaatta cattgacaaa ggaagtctca gaaatgccca
301 gaatagactt tgttctcctt ttatgtctca aagtactggc tggttttgtg tgtcaacttg
361 acacaagctg gagttatcac tgagaaagga gccttcctta aggaaatccc tccacaagat
421 acacctgtaa gcagtttctc aattagtgat caaggcagag ggcccgttgt gggtggtgac
481 atcactggac gggtggtctt gggttccata taaaagcaag ctggccaggc gtggtggagc
541 acgcgtttaa tcccagcact tgggaggcag gggcaggtgg gtttctgagt tcaaggccag
601 actggtcttc aaagtgagtt ccaggacagc cagggctata cagagaaacc ctgtctagaa
661 aaaccaaaaa ganaaaaaaa aaaaaaaaaa aaaaaaacaa gctgaccaag ccaggggaac
721 caaacagtaa gtaacatc
//