LOCUS BG070119 747 bp mRNA linear EST 12-MAY-2010
DEFINITION H3083H05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3083H05 3', mRNA sequence.
ACCESSION BG070119
VERSION BG070119.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 747)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG070119.1.
Other_ESTs: H3083H05-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3083 row: H column: 05
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..747
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3083H05-3"
/db_xref="taxon:10090"
/clone="H3083H05"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 192 a 193 c 173 g 189 t
ORIGIN
1 aagcttggaa aagacattta atcaatctct tgatgtctgt gaattactaa taaagctacg
61 cacatgttca cagaatgttt gtggctacca acatgaatga tacgagccat taatttttgg
121 gaacatctcc caagctaata gtctccatct cccagcagac tcagctcggg ctttgcttcc
181 tccacatcct tccagtaagg aagcagccac ggcagagatg agaccttctt gtccagcagt
241 ggacagaggt ttaagaacag aaactcattt cccattggtg ataaatgcag gccatccgat
301 aaataggatg agaagtcctg gctgtccttc tgcatcaggg tccacaggtc aaggacatca
361 gtcccacagt ctcgggctac ttgtaaacac gcatttgcat attcaccaac aactgagttc
421 aggcggttta atttgcaacc cttcaggacg cactccttct cccaggcggc ttcgcagagc
481 gggggtggcg tgatagggat gacccgctcc ctggggacgt ccacagacct caggtactgc
541 accatgtccc tcaggtttgc gctgtactca tccaagggga cgtgctgctt ggggttctca
601 tctttgagtg aagagtcatt ggcaccaaag aagattgtaa cggccactgg gttttccatc
661 ccaggccctt tcctgataag tcttgggagg ataatcttgg cccatctggt gttataacct
721 gaaaatccac gattcagaac atcacac
//