LOCUS BG070110 799 bp mRNA linear EST 12-MAY-2010
DEFINITION H3083G06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3083G06 3', mRNA sequence.
ACCESSION BG070110
VERSION BG070110.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 799)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG070110.1.
Other_ESTs: H3083G06-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3083 row: G column: 06
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..799
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3083G06-3"
/db_xref="taxon:10090"
/clone="H3083G06"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 217 a 204 c 165 g 213 t
ORIGIN
1 ggtttttcaa gacagggttt ctcagtataa ccctggctgt cctggaactc acttttgtag
61 accaggatgg cctcaaaact cagaaatccg cctgcctctg cctccccagt gctgggatta
121 aaggtgtgca ccaccacact cagctagctg gactctgtta cataaactac tggggcaaaa
181 ctgctggccc ctggaggaga actagtggct tttaagaggg caacagttgt cactcttgga
241 tacgaagact agtgcttacc aagcacaggc atggttagac attgcagaag actctaaata
301 aacttgaatg agtttaattc ctcagccaca cttggcccaa ctgtcaagac agcataaaag
361 tgtgagaact aatcaagctt atctctgcct attacaaatg ttaagctggc tgcctcttgt
421 aacttttcct tcactacatt aagcctacag ccaacaagtc tgcaataaag caactatggt
481 ggccgcaatg acacaatcag ccaagtcctt tacccattat ttttatgagt gttttgcctt
541 cctataagta tatgtaccac atgcatgtct ggtgtccaag gtcagccatg tcagatccat
601 gtaactggaa ttacagatgg ctgtgagcca ccacatgtgt gcttagaacc aagtccaagt
661 cctatgcaaa agcaactctc caatcttaaa actttgttga gataggggtc tcaccacgtc
721 cccatagctt gccccgaact caagaggtct gcttgccttc gcctctggag tgctgtgatc
781 aaagatctgt gcctcactc
//