LOCUS BG069968 830 bp mRNA linear EST 26-JAN-2001
DEFINITION H3083B07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3083B07 3', mRNA sequence.
ACCESSION BG069968
VERSION BG069968.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 830)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Other_ESTs: H3083B07-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3083 row: B column: 07
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..830
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3083B07-3"
/db_xref="taxon:10090"
/clone="H3083B07"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 201 a 173 c 177 g 279 t
ORIGIN
1 ctgtatactt attgttttga ttttatatgc taagaggatt ttcttttctg gtccaggcta
61 cctggtgtgc tggagtcttc ttgataggca tcttcttatt taggtttagg gaaattttct
121 tccataagtc tataattctg ttacaatatt ttctatgcct ttcacctgtg tttcttctcc
181 ttcctctatt actgttaatc ttaaatttgg tgtttcatag tgtcccagat ttcctgggta
241 ttttgtggca agaggttttt agatcttctt tgcctggaga taactatttc ttctacaatc
301 tcataatgtc tgagattctc ttttccatat tgtattctgt cggtgaggtt tgcatcttgt
361 ttgagtctct aaatcctttc atttccagat ttccctcagt ttaggctttt caagacctat
421 ttttaatgat ggctcttaaa cacttcaaac tcctttttag cccaccaccc accagaggta
481 gtggaaaaga aaggatacat ggaagtggac ctgtttagaa aggttctttg gagcaactct
541 tgtctgtgtc atctggaaat gggcagttca gttcacaggt cagcagcggc agcttgatcc
601 cctcacaaac gcttcacagt ctagtttggt agatttcaga taacaaacag gaatcagcag
661 cggtggcact acctagcagg gacagccagg cctcagcctc agctagagtc agcaggaggg
721 accggtgagg acaccaggga aagttctcca ctgtgcctct ctcagagagg tgaagatcag
781 caaagacatg agatgcgaag tgttgaacag ctagctctag aagcaagcta
//