LOCUS BG069855 708 bp mRNA linear EST 12-MAY-2010 DEFINITION H3080H03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3080H03 3', mRNA sequence. ACCESSION BG069855 VERSION BG069855.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 708) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG069855.1. Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3080 row: H column: 03 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..708 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3080H03-3" /db_xref="taxon:10090" /clone="H3080H03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 176 a 185 c 202 g 145 t ORIGIN 1 tgacgaatac actttgaagc tcatgcagtg ctggaaggag gaactgggaa cccaggcaac 61 caccattcta agtgtgtggg ggtggctggg gtatccagat aggaatgggg aaacagaact 121 ggcagagaca gtctggactc agatctacca cagagcctag gcccaggccc ccagggtgtc 181 accttcttcc tccccaaagg actagagaat tccagagatt ccaaaagacc tcggaggaga 241 tggtgggaag cctgagttag gttaatcact cctgcgaatg gctcatttgg atcttatgtc 301 cactgggtca tagatgtagc agcccacatc gccaatgttc acaccacaag ggaggttgtc 361 cacacattct cataggtcca gtgtctgggg tcagattgcc cacctttggg gccaaacagg 421 tatccatagc aggggatgtg gcagtaaggc atgccatcat gctccgcatg actcccagca 481 gtcagggtct tcctgcaacg ttggcacctc aggcagggtc ggtgccaatt tcaaaaaaga 541 caggatcccc acatccaggg cacagtgaag tctcaccagt gaatgtcttc aagtagggag 601 gccctttaat gggaagaggc tccggatagg gctgtggtgg gggccaggcc ctgagcaggg 661 gcttctagcc ccacctgtac atggatggca ctgtcctctc ccaaaccc //