LOCUS BG069727 707 bp mRNA linear EST 12-MAY-2010 DEFINITION H3079D12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3079D12 3', mRNA sequence. ACCESSION BG069727 VERSION BG069727.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 707) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG069727.1. Other_ESTs: H3079D12-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3079 row: D column: 12 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..707 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3079D12-3" /db_xref="taxon:10090" /clone="H3079D12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 160 a 180 c 224 g 143 t ORIGIN 1 aagcacaggc tatacagaca attatttact tataatcttg gccctttggg ggtgggccaa 61 aggtctgggc ccagcatgca cccccacttc tttggggccc aatccccagc tctgctaggt 121 ccacaggggc cagagtgtca ggccagtggc ggtggaggta gcagcaggag tatagtggag 181 agtacagagc cacacgggat agacagaggc aagggcatgg ggacagtaac caggacccag 241 agctgagtcc aaggaggcct ggaaggcttt gacctgtccg tcgggctgag cctcaaagca 301 ggcggcaggc attgcccaca ctgttggctg aggtgtcgag atcgtggctg tatggggatt 361 cccagtctct caggaaaaca gcctccagct gactgcgcaa ccaccatgcc cgttctgtgt 421 caccagcagg gaggtgcctg ctgtctccgt gaagtagctt ccagaccagt tggaggttcc 481 aatgtatgag gcacgttcag tcaccatgta cttgttgtgg ttgacacggg cataggggat 541 tcgggcctgg gactcatccg taggaccaca aacagtttca cctggatgtc ggagtgagta 601 tggttgtcat gaagtgcagc cagggagagc aggaaggacc gcatcgatgg atcagagtgt 661 ccccagcagc tgatgagcag gcgcactttg acgcctcgtt catacgc //