LOCUS BG069697 694 bp mRNA linear EST 12-MAY-2010
DEFINITION H3079B06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3079B06 3', mRNA sequence.
ACCESSION BG069697
VERSION BG069697.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 694)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG069697.1.
Other_ESTs: H3079B06-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3079 row: B column: 06
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..694
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3079B06-3"
/db_xref="taxon:10090"
/clone="H3079B06"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 183 a 163 c 153 g 195 t
ORIGIN
1 aaatggccac ctgtgccagt taaatagctt tattcaaaag aaaaccaaac taaacaacaa
61 taattaaaaa caaatcttct tactatggca agtttttaga gctgggattc tcctgacaga
121 aacagactcc tggcatctcc taggtatcat gagatgtgga cattctccat caaacaactg
181 aatttctgag ctgtccccat gctattgaga aagctactag ttacattgtt aaaaatccct
241 cttacaacca ggtcattgct gaaggcagat gtcacaacac tagcaaacag agaatgcaga
301 aattactttg gtaggcaatt cccaaagtca aagaatatat ttttagtctc cttggtaatc
361 catctgtcca attgcatagt tttgtttaaa tgtacaaaac ccaccccttg ctgcattcac
421 atggggcttc tggaagttgg agtcacccct tgctgcattc atgcggggct tctagaagtt
481 ggagtcaccc cttgctgcat tcacgcgggg cttctggaag ttggagtcac cccttgctgc
541 attcacgtgg ggcttctgga agttggagtc accccttgct gcattcatgc ggggcttctg
601 gaagttggag tcaccccttg ctgcattcac gtggggcttc tggaagttgg agtcacccct
661 tgctgcactc atgcggggct tctggaagtt ggag
//