LOCUS BG069659 632 bp mRNA linear EST 12-MAY-2010
DEFINITION H3078G02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3078G02 3', mRNA sequence.
ACCESSION BG069659
VERSION BG069659.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 632)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG069659.1.
Other_ESTs: H3078G02-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3078 row: G column: 02
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..632
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3078G02-3"
/db_xref="taxon:10090"
/clone="H3078G02"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 256 a 106 c 83 g 187 t
ORIGIN
1 cagaaaatct gaaacattta atggatgaaa tgcacacaca atataaataa gaaagtaact
61 tggataaaaa agattctgaa gaggaactga tcaaaacttc aatatatgaa taacaatata
121 tgcttgcatt acagtgtata gttatgagtt aagtgtattt agatttgtaa agaaattgaa
181 ctataactac tgcaagatta ttccaagaat attcttcaaa tgtacaataa aaacacaaag
241 ccttgtgcat gttaaacatg actatcacgc caggcaaaca agtccttaac cacgttttaa
301 aatgggttca tttataccat ttaaacacac acaaaaaaag gctttcaaag gcattaaaac
361 atagacttca gtgtccaact cacattttga cctctatgct ttcacattta atagttgcat
421 tttgaatgta taaactaaaa attttaaatc tttctcaaaa agtttatgtc tgcatgtctt
481 ataagaatta tatttgagat ctctaatgct aagaactaaa acatgtactt gaaacttaaa
541 agaaaaaaat cataagttca caatcatgtc cactacaacg tccattaagt gacttaaaag
601 caaacaagtc aaatgcccag gtccttcctc ag
//