LOCUS BG069644 804 bp mRNA linear EST 12-MAY-2010 DEFINITION H3078E11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3078E11 3', mRNA sequence. ACCESSION BG069644 VERSION BG069644.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 804) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG069644.1. Other_ESTs: H3078E11-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3078 row: E column: 11 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..804 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3078E11-3" /db_xref="taxon:10090" /clone="H3078E11" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 184 a 202 c 213 g 205 t ORIGIN 1 gcagatctgt taaaaggttt aatactgaaa attacatatt aaatacagtc acataggcag 61 cagcagttta ttaagcatca agcctcttgt gtgtgaggtg gcagagtgtg gtactagttt 121 ccaagaagcc agaattaagt caggcgagct gtgactgctg tctctgcagc acacattagt 181 aatgggattg ccatcccttt ccctctccaa cagcgccccc tgggagacct gtgaactaga 241 accttgtaga tctgcctgga cctgaagcca aagtgagcgg tccttctctt ttggaaggag 301 aatggtctgg cttgacacct gaacaggagc agtggttaga attgaaacta cttgcacgtg 361 aaggcattgg agatgtcagt gctctgccct gaactaaagg gtggaagcag cttgtcacag 421 gttcttttgc ccactggaga cacctggcgc tcaggaagat gcttgccggg ctggtctcca 481 gcagaagcca caagcatggc tcctgactac tgtttttgct cttgataaaa agaatcgatg 541 atatctttgt acttctccag aaccgtgagc cgtttcaatt tcatggtggg tcccagttct 601 ccacctgaaa tggagaagtc acgttggaga atggcccact tctggatgtg gtagggccgg 661 gctgcggcgt tcgcgttcac cctctggatc ccttcgtgaa tggcctggta cacagcctcg 721 tctctctgcc ccacgatctc ggagacggtg ctggccttgc tgcccacccg ctggcagaac 781 tccacagctt gctcggtcag gctg //